- 中文名稱
Nicotinamide-N-Methyltransferase Inhibitor Screening BioAssay Kit (NNMT)
- 英文名字
- Nicotinamide-N-Methyltransferase Inhibitor Screening BioAssay Kit (NNMT)
- 供應商
- USBiological
- 產品貨號
- 301251
- 產品報價
- ￥1/100Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy. Intended Use:For screening, studying, and characterizing potential inhibitors of NNMT.Specificity:Species Crossreactivity: mammalianTest Principle:This NNMT Inhibitor Screening BioAssay Kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe generating enhanced fluorescence signal that can be measured at Ex/Em=392/482nm. In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence.?This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.Kit Components:NNMT Assay Buffer, 1x22ml ?NNMT Enzyme, 1x50ul ?S-Adenosylmethionine (SAM), lyophilized, 4x vial ?Nicotinamide, 3x1.5ml ?Enzyme-I, 1x200ul?Enzyme-II, lyophilized, 1x vial?1-Methylnicotinamide (MNA), 150mM, 1x20ul ?Thiol Detecting Probe, DMSO, 1x200ul ?SAM Reconstitution Buffer, 1x500ulStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對Nicotinamide-N-Methyltransferase Inhibitor Screening BioAssay Kit (NNMT)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Nicotinamide-N-Methyltransferase Inhibitor Screening BioAssay Kit (NNMT)產品后將其置于-70°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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