- 中文名稱
p53, BioAssay ELISA Kit (Human)
- 英文名字
- p53, BioAssay ELISA Kit (Human)
- 供應商
- USBiological
- 產品貨號
- 301261
- 產品報價
- ￥1/100Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- This p53 Enzyme-Linked Immunosorbent Assay (ELISA) BioAssay Kit is an in vitro assay for the quantitative measurement of native and recombinant human p53. The p53 protein is encoded by the TP53 gene. It is composed of 393aa but its apparent molecular mass is 53kD. p53 binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle, or causing arrest in G1 when the genome is damaged. It acts as a pivotal suppressor of inappropriate cell proliferation. By initiating suppressive effects through induction of apoptosis, cell senescence, or transient cell-cycle arrest, p53 plays an important role in cancer suppression, developmental regulation, and aging. p53 is found in increased amounts in a wide variety of transformed and tumor cells, where its concentration is increased 5-1000 fold over the concentration in normal cells. Meanwhile, p53 is also frequently mutated or inactivated in about 60% of cancers. Sample Type:Cell lysate and cell culture supernatant. Serum and plasma. Intended Use:Quantitative measurement of native and recombinant p53.Sensitivity:0.1ng/mlRange:0.2-25.6ng/mlSpecificity:Recognizes p53. Species Crossreactivity: human. This ELISA kit shows no cross-reactivity with other relevant proteins.Test Principle:The p53 ELISA BioAssay employs an antibody specific for human p53 coated on a 96-well plate. Standards and samples are pipetted into wells and p53 present in the sample is bound to the wells by immobilized antibody. Wells are washed and a biotin-labeled human p53 specific detection antibody is added. After washing away unbound detection antibody, a streptavidin HRP-conjugate is added to the wells. The wells are again washed, a TMB substrate solution is added, and color develops in proportion to the amount of bound p53. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. Recovery within this range is 86.3%. The intra-assay reproducibility as measured by the coefficient of variation (CV) is <8% and inter-assay has CV <12%.Kit Components:Plate Coated with p53 Ab, 1x96 wellsAssay Diluent, 1x27ml Wash Buffer A, 10x, 1x10ml Wash Buffer B, 10x, 1x10ml Wash Buffer C, 10x, 1x10ml rh p53 Standard, 3x vials Detection Antibody, 100X, 1x110ul Streptavidin-HRP Conjugate, 100X, 1x120ul TMB Substrate, 1x11ml Stop Solution, 1x11mlPlate Sealer, 2x sealers Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對p53, BioAssay ELISA Kit (Human)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到p53, BioAssay ELISA Kit (Human)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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