Caspase-3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase-3 proteolytically cleaves and activates other caspases，as well as relevant targets in the cells （e.g.，PARP）. The affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
應用類型
Western blotting （1:50 -1:500 dilutions） and Immunohistochemistry （1:10-1:20 dilutions）. However， the optimal concentrations should be determined individually. The antibody preferentially recognizes the p17 fragment of the active caspase-3 in samples from human， mouse， and rat origins
免疫原
Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Caspase-3 （Active）蛋白發生免疫反應.
保存建議
提供的Caspase-3 （Active）抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS緩沖液中.請將該Caspase-3 （Active）抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
