Caspase-9 is one of the most important caspases among the caspase family members. Caspase-9 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis. The processed caspase-9 consists of large （32-35 kD） and small （10 kD） subunits which associate to form an active enzyme. Activated caspase-9 in turn cleaves and activates caspase-3，one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects proform （~46 kDa）， intermediate （~33-36 kDa）， and the fully cleaved （~20-25 kDa） caspase-9.
免疫原
Synthetic peptide surrounding amino acid 290 of human caspase-9
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、倉鼠、牛、兔、豬種屬來源的Caspase-9蛋白發生免疫反應.
保存建議
提供的Caspase-9抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Caspase-9抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
