During apoptosis，Apaf-1 （apoptosis protease activating factor 1） binds to cytochrome c （Apaf-2），a reaction that requires dATP，and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase-9，and activates caspase-9 （Apaf-3）. Activated caspase-9 in turn cleaves and activates caspase-3，one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 is ubiquitously expressed in human tissues.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 130 kDa protein， corresponding to the apparent molecular weight of Apaf-1 on SDS-PAGE immunoblots.
免疫原
Synthetic peptide surrounding amino acid 1161 of human Apaf-1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、牛、兔、猴、馬種屬來源的Apaf-1蛋白發生免疫反應.
保存建議
提供的Apaf-1抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.05% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Apaf-1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
