PARP，a 116 kDa nuclear poly （ADP-ribose） polymerase，appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like Caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP，the cleavage occurs between Asp214 and Gly215，which separates the PARP amino-terminal DNA binding domain （24 kDa） from the carboxy-terminal catalytic domain （89 kDa）. PARP helps cells to maintain their viability； cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.
應用類型
The antibody performs well on Western blot （1:500-1:2000） and Flow Cytometry studies （1:200-1:400）.
免疫原
A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. The antibody only recognizes full-length form （p116） of PARP-1.
來源宿主
小鼠
反應性
經檢測驗證，可與人種屬來源的PARP蛋白發生免疫反應
保存建議
提供的100 ulPARP抗體（7A10）產品為無色溶液其濃度為1 mg/ml溶解于包含有50%甘油， 0.01% BSA、150 mM NaCl、0.03%疊氮化鈉和10 mM HEPES緩沖液（pH 7.5）中.請將該PARP抗體（7A10）產品置于-20 °C冷凍保存，建議分裝使用以避免反復凍融.
