Cytochrome c （m.w. 12，500） is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis，prior to caspase activation，DNA fragmentation，and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases
應用類型
Western blotting （0.5-4 ug/ml） and immunoprecipitation （10-20 ug/ml）. The cytochrome c antibody detects the 12.6 kDa cytochrome c from human， mouse， and rat samples. Jurkat cell lysate， NIH3T3 cell lysate， and rat kidney tissue lysate can be used as positive controls.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c
