The Bcl-2 (B-cell leukemia/lymphoma 2) family proteins function to either inhibit (Bcl-2 and Bcl-XL) or promote (Bax and Bak) apoptosis. Bax contains the conserved Bcl-2 homology 1 (BH1) and 2 (BH2) domains that allow for its homodimerization or heterodimerization with Bcl-2. When Bcl-2 is in excess,apoptosis is inhibited. However,if Bax levels increase in response to a death signal,the cell is pushed toward death.
應用類型
Western blot analysis (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml). However, the optimal conditions should be determined individually.The antibody detects a 20 kDa protein, corresponding to the apparent molecular weight of Bax on SDS-PAGE immunoblots.
免疫原
Synthetic peptide corresponding to amino acids near the N-terminus of human Bax
來源宿主
兔
反應性
經檢測驗證,可與人、小鼠、大鼠、猴種屬來源的Bax蛋白發生免疫反應.
保存建議
提供的Bax抗體產品為100 ug 無色溶液,其濃度為0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)緩沖液中.請將該Bax抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.