Mcl-1 （Myeloid cell leukemia-1） is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1，or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-B1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells，Mcl-1 interacts with another Bcl-2-related protein，Bax，and prolongs cell viability after treatment with different apoptotic reagents.
應用類型
Western blot analysis （0.5-4 ug/ml）， immunoprecipitation （10-20 ug/ml）， and Immunohistochemistry （10-20 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 131 of human Mcl-1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、猴種屬來源的Mcl-1蛋白發生免疫反應.
保存建議
提供的Mcl-1抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Mcl-1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
