p53，a 53 kDa protein，binds to a DNA consensus sequence，the p53 response element，and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle，or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells，principally due to the increased half-life （4 hrs） compared to that of wild-type （20 min）. p53 localizes in the nucleus，but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression，but may not always be necessary for apoptosis.
應用類型
Western blot analysis （0.5-4 ug/ml）. It detects p53 in samples of human， mouse and rat origins. Other applications have not been determined.
免疫原
Synthetic peptide corresponding to residue surrounding amino acid 375 of human p53
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、狗、兔種屬來源的p53蛋白發生免疫反應.
保存建議
提供的p53抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，請將該p53抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
