Fas-associating death domain containing protein （FADD），also known as mediator of receptor induced toxicity （MORT1），is a 23 kDa cytosolic protein with an apparent molecular weight of 28 kDa on SDS-PAGE. The protein is involved in both Fas and TNF-R induced cell death pathway. FADD specifically binds to Fas to initiate the apoptotic signalling cascade. Overexpression of FADD induces apoptosis.
應用類型
Western blot analysis （0.5-4 ug/ml）and can also be used in immunoprecipitation. However， the optimal conditions should be determined individually. The antibody detects an ~28 kDa band， corresponding to the expected molecular mass of FADD on immunoblots.
免疫原
Synthetic peptide surrounding amino acid 128 of human FADD
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、猴種屬來源的FADD蛋白發生免疫反應.
保存建議
提供的FADD抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該FADD抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
