Lamins are classified into A- and B- type isoforms. Specifically，four distinct lamin isoforms have been identified: A，B1，B2，and C. Lamin A and C are alternatively spliced products of a single gene，whereas lamin B1 and B2 are encoded by distinct genes. During mitosis，lamins are phosphorylated by p34cdc2 and solubilized prior to chromosome condensation and nuclear breakdown. A direct role for lamins in chromosome condensation is suggested by the fact that lamin B binds to specific DNA sequence motifs termed matrix attachment regions （MARs）. Mars mediate the interaction of chromatin with the nuclear matrix. Importantly，some of the same processes that involve mitotic chromosome condensation and nuclear envelop breakdown are also activated during apoptosis. It appears that chromosome condensation during apoptosis is accompanied by degradation of lamin protein. Lamin degradation occurs prior to DNA fragmentation and this degradation is useful as an apoptotic marker.
應用類型
Western blotting （0.5-2 ug/ml）， immunoprecipitation （5 ug/ml）， immuno-fluorescence （10-15 ug/ml）， and ELISA （0.2-1 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
Recombinant human Lamin B
來源宿主
小鼠
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Lamin B1蛋白發生免疫反應.
保存建議
提供的Lamin B1抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有1 mg/ml BSA和1.5 mM 疊氮鈉和50%甘油.的PBS緩沖液中.請將該Lamin B1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
