Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as many other types of diseases. Recently，a human DNA fragmentation factor （DFF45） was identified. DFF is composed of two subunts: DFF40 and DFF45. Cleavage of DFF45，which is mediated by caspase-3，leads to DFF40’s activation as a nuclease. Activation of DFF40 can lead to DNA fragmentation，a hallmark of apoptosis.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects the 45 kDa and 28 kDa proteins， corresponding to the apparent molecular weight of DFF45/ICADL， and ICADS in immunoblots.
免疫原
Synthetic peptide mapping to the N-terminus of mouse DFF45
來源宿主
兔
反應性
經檢測驗證，可與小鼠、大鼠種屬來源的DFF45蛋白發生免疫反應.
保存建議
提供的DFF45抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該DFF45抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
