DFF is composed of two subunits: DFF40/CAD and DFF45/ICAD. Human DFF45 and its mouse homologue ICAD are the inhibitors of DFF40 and CAD，respectively. Cleavage of DFF45，which is mediated by caspase-3，leads to DFF40’s activation as a nuclease. Activation of DFF40/CAD can lead to DNA fragmentation，a hallmark of apoptosis. DFF40 mRNA is expressed in limited number of human tissues: pancreas，spleen，prostate，and ovary.
應用類型
Western blotting （0.5-4 ug/ml） and Immunohistochemistry （2-8 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects a 43 kDa DFF40/CAD of human， mouse and rat origins. Mouse kidney tissue lysate can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 208 of mouse DFF40
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的DFF40蛋白發生免疫反應.
保存建議
提供的DFF40抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該DFF40抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
