Apoptosis can be induced by certain cytokines including TNF and Fas in the TNF superfamily through interaction with their death domain （DD） containing receptors，TNFR1 and Fas. A member of the TNF receptor family was recently identified and designated DR3 （also called Wal-1，Apo3，TRAMP and LARD）. The ligand for this novel death receptor has been defined as TWEAK （also termed Apo3L）. Like TNFR1，overexpression of DR3 triggers apoptosis and NF-kB activation. Activation of DR3 results in recruitment of TRADD （a DD containing adapter molecule）. TRADD associates with FADD，TRAF2，and RIP to activate the signaling complex for NF-kB activation. DR3 activates apoptosis through TRADD，FADD，and caspase-8. DR3 is highly expressed in the tissues enriched in lymphocytes including peripheral blood leucocytes （PBL），thymus and spleen.
應用類型
Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to residues surrounding amino acids 378 of mouse DR3
