Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1，TNFR2，CD27，CD30，and CD40. Binding of Fas-ligand （FasL） to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic “death domain” that is responsible for transmitting signal for apoptosis.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The purified antibody detects a 55 kDa of glycosylated Fas， a 35 kDa native form of Fas and a 29 kDa of cleaved Fas fragment on SDS-PAGE immunoblots.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 32 of mouse Fas
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Fas/Apo1蛋白發生免疫反應.
保存建議
提供的Fas/Apo1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Fas/Apo1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
