Calcineurin is a calcium/calmodulin-dependent serine/threonine protein phosphatase. The active enzyme is a heterodimer consisting of a catalytic A subunit of 61 kDa and a myristylated regulatory B subunit of 19 kDa that binds calcium. The catalytic A subunit consists of an amino terminal catalytic domain，followed by calmodulin and B subunit binding sites，and a caboxyl-terminal autoinhibitory domain. Calnineurin is the target of immunosuppressant drugs such as cyclosporin A and FK506. Calcineurin controls the translocation of nuclear factors of activated T cells （NFAT），a family of transcriptional activators that control the expression of cytokine genes essential to immune response，from the cytosol into the nucleus of activated T cells. Calcineurin has been proposed to play an important role in mediating calcium-activated cell death.
應用類型
Western Blotting （1-4 ug/ml） and for immunoprecipitation （10-20 ug/ml）. However， the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 61 kDa band， corresponding to the expected molecular weight of Calcineurin A on immunoblots. A 32 kDa band can also be detected in human samples， presumably to be the cleavage product of Calcineurin A..
免疫原
Synthetic peptide corresponding to residues surrounding amino acids 267 of human Calcineurin A
