Human Hsp27，mouse Hsp25 and B-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of as many as 8-40 Hsp27 protein monomers in cells and data suggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines，glycines or aspartates，have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate that Hsp27 is also a negative regulator of cytochrome c-dependent activation of procaspase-3.
應用類型
Western blotting （1-2 ug/ml）， immunoprecipitation （5-10 ug/ml）， immunocytochemistry （10-15 ug/ml）， and ELISA （1 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects a 27 kDa protein， corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots， in samples from human， monkey， dog （weakly） and pig （weakly） origins.
免疫原
Recombinant human Hsp27
來源宿主
兔
反應性
經檢測驗證，可與人/猴種屬來源的Hsp27蛋白發生免疫反應.
保存建議
提供的Hsp27抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Hsp27抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
