Hsp70，one class of stress proteins，is comprised of multiple members，all of which bind ATP in vitro，but are localized within different intracellular compartments. These include: a） the constitutive Hsc70 （or cognate） present within the cytosol/nucleus； b） the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus； c） the constitutive glucose regulated 78 kDa （or BiP） protein present within the lumen of the endoplasmic reticulum； and d） the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions，but is expressed at high levels in the cell experiencing stress. Consequently，detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell.
應用類型
Western blot analysis （1 ug/ml）， immunoprecipitation （5-10 ug/ml）， Immunohistochemistry （5-20 ug/ml， frozen & paraffin）， and FACS analysis. However， the optimal conditions should be determined individually. The antibody recognizes a 70 kDa protein， corresponding to the apparent molecular weight of the inducible Hsp70 on SDS-PAGE immunoblots. This antibody has no reactivity with the constitutive Hsc70 （Hsp73）.
免疫原
Recombinant human Hsp70
來源宿主
小鼠
反應性
經檢測驗證，可與人、小鼠、大鼠、猴、倉鼠、兔、牛種屬來源的Hsp70蛋白發生免疫反應.
保存建議
提供的Hsp70抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有1 mg/ml BSA和1.5 mM 疊氮鈉和50%甘油.的PBS緩沖液中.請將該Hsp70抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
