Hsp90 （also known as Hsp82， Hsp83， or Hsp89）， a 90 kDa protein， is the most abundant protein in the cytosolic fraction in many cell types. The high level of Hsp90 in many cell types suggests that Hsp90 may play a general role in the cell， but little is known about its general function. Hsp90 has no known enzymatic activity， and thus it has been presumed that Hsp90 may function through protein-protein interactions. Hsp90 exists in a dimeric form and has been observed to bind to several cellular proteins such as retro-virus kinases （PP60v-src）， steroid receptors， heme-regulated protein kinase， actin and tubulin. In this regard， Hsp90， like Hsp70， may function as a “molecular chaperone”. Hsp90 exists as two isoforms referred to as Hsp86 and HSP84 in murine cells or Hsp90α and Hsp90β in human cells.
應用類型
Western blot analysis （1-2ug/ml）， immunoprecipitation （4-8 ug/ml） and Immunohistochemistry （10-20 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes a 90 kDa protein， corresponding to the apparent molecular weight of Hsp90 on SDS-PAGE immunoblots. This antibody detects both Hsp90 alpha and beta.
免疫原
Achlya ambisexualis Hsp90
來源宿主
小鼠
反應性
經檢測驗證，可與人、小鼠、大鼠、猴、倉鼠、兔、牛種屬來源的Hsp90蛋白發生免疫反應.
保存建議
提供的Hsp90抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有1 mg/ml BSA， 50%甘油和0.2%硫柳汞的PBS緩沖液中.請將該Hsp90抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
