MAP kinase-activated protein kinase-2 （MAPKAPK-2），also known as p45 hsp27 kinase，is a 45-54 kDa serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MAPKAPK-2 is activated in response to stress，IL-1 and TNF，possibly catalyzed by p38/Hog-dependent phosphorylation. One of the major substrates of MAPKAPK-2 is hsp27，which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. Two isoforms are produced due to alternative splicing of the same gene which differs in their C-terminals. This antibody recognizes both isoforms.
應用類型
Western blot analysis （0.5-4 ug/ml） and immunoprecipitation. However， the optimal conditions should be determined individually. The affinity purified antibody detects both 43 and 60 kDa isoforms， corresponding to the apparent molecular mass of MAPKAPK-2 on SDS-PAGE immunoblots. These 43 kDa and 60 kDa bands can be specifically inhibited by the relevant peptide.
免疫原
Synthetic peptide surrounding amino acid 365 of mouse MAPKAPK-2
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、狗、牛種屬來源的MAPKAPK-2蛋白發生免疫反應.
保存建議
提供的MAPKAPK-2抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該MAPKAPK-2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
