AMPK beta-1 chain is a regulatory subunit of the AMP-activated protein kinase （AMPK）. AMPK is a heterotrimer consisting of an alpha catalytic subunit，and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses，AMPK is activated，and thus phosphorylates and inactivates acetyl-CoA carboxylase （ACC） and beta-hydroxy beta-methylglutaryl-CoA reductase （HMGCR），key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
應用類型
The antibody can be used for ELISA （0.25 ug/ml）， Western blotting （0.5 – 2.5 ug/ml） and Immunohistochemistry （2.5-5.0 ug/ml）.
免疫原
Synthetic peptide selected from the N-terminal region of human PRKAB1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的AMPKB蛋白發生免疫反應.
保存建議
提供的AMPKB抗體產品為100 ug 無色溶液，其濃度為0.25 mg/ml，溶解于包含有0.09% （W/V） 疊氮鈉的PBS緩沖液中.請將該AMPKB抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
