Protein kinase A (PKA) also known as cAMP-dependent protein kinase,is a 40 kDa protein kinase that phosphorylates serine or threonine residues in target proteins in response to elevated levels of cAMP. It has been suggested that the complex structure of PKA may be necessary for it to accomplish such diverse functions.
應用類型
Western blot analysis (0.5-4 ug/ml) and in immunoprecipitation (20-30 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 11 of the catalytic subunit of human PKA alpha isoform
來源宿主
兔
反應性
經檢測驗證,可與人、小鼠、大鼠、牛種屬來源的PKA蛋白發生免疫反應.
保存建議
提供的PKA抗體產品為100 ug 無色溶液,其濃度為0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)緩沖液中.請將該PKA抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.