Human Raf1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of Mek1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase （Mek1）. It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. Raf1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in Raf-1. Raf-1 is activated upon phosphorylation by protein kinase C，Src and ceramide-activated protein kinase. Raf-1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain （CR1），phosphorylation site domain （CR2） and the catalytic domain （CR3）. Raf-1 physically interacts with Ras，RapIB，MEK1，the 14-3-3 family of proteins and the molecular chaperones hsp90 and p50.
應用類型
Western blot analysis （1-4 ug/ml）， immunoprecipitation （15-25 ug/ml） and Immunohistochemistry （10-20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots. It is non cross-reactive with Raf-A and Raf-B.
免疫原
Synthetic peptide mapping to the C-terminus of human Raf-1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Raf1蛋白發生免疫反應.
保存建議
提供的Raf1抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Raf1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
