Wee1 is a nuclear tyrosine protein kinase. The protein plays a role in suppressing eukaryotes’ entry into mitosis by inactivating cdc2 kinase by inhibiting tyrosine phosphorylation. The predicted molecular weight of the kinase is 72 kDa. The kinase possesses tyrosine kinase activity，but structurally belongs to the serine/threonine protein kinase family.
應用類型
Western blot analysis （0.5-4 ug/ml） and in in immunoprecipitation （5-10 ug/ml）. However， the optimal conditions should be determined individually. The affinity-purified rabbit antibody recognizes Wee1 protein from human， mouse and rat samples. Mouse small intestine tissue lysate （Cat.# 2601-100） can be used as a positive control
免疫原
Synthetic peptide mapping to the C-terminus of human Wee1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Wee1蛋白發生免疫反應.
保存建議
提供的Wee1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Wee1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
