Tumor necrosis factor receptor 1 and 2 （TNF-R1 and TNF-R2） are 55 and 75 kDa. While TNF-R1 and TNF-R2 share 28% sequence homology in the extracellular domains，their intracellular domains lack sequence homology，suggesting that they differ in their internal signal transduction pathways. TNF-R1 contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD （TNF-R1 associated death domain protein），and subsequent interactions with FADD. TNF-R1 can also activate the transcription factor NFkB via TRAF2 （TNF receptor associated factor 2）. The cytoplasmic domain of TNF-R1 can directly interact with Jak kinase，thereby activating the JAK/STAT signal transduction cascade.
應用類型
Western blotting （0.5-4 ug/ml） and in Immunohistochemistry （10-20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects cdc42 of human， mouse， rat， and bovine origins.
免疫原
Synthetic peptide surrounding amino acid 32 of human TNF-RI
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、倉鼠、牛、兔、猴種屬來源的TNF-R1蛋白發生免疫反應.
保存建議
提供的TNF-R1抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該TNF-R1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
