PARP，a 116 kDa nuclear poly （ADP-ribose） polymerase，is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215，which separates PARP’s N-terminal DNA binding domain （24 kDa） from its C-terminal catalytic domain （89 kDa）. It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
應用類型
Western blot analysis （0.5-4 ug/ml） and immunocytochemistry （10-20 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes only the large fragment of PARP （89 kDa） and does not react with the full length PARP. Camptothecin treated Jurkat cell lysate can be used as a positive control.
免疫原
Synthetic peptide correspond to the N-terminal residues of the catalytic domain of human PARP
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的PARP （Cleaved）蛋白發生免疫反應.
保存建議
提供的PARP （Cleaved）抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS緩沖液中.請將該PARP （Cleaved）抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
