Hexokinases phosphorylate glucose to produce glucose-6-phosphate，thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase，one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase，this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non-insulin dependent diabetes mellitus （NIDDM），also called maturity onset diabetes of the young，type 2 （MODY2）； mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy （PHHI）.
應用類型
The antibody can be used for ELISA （0.25 ug/ml）， Western Blotting （0.5 – 2.5 ug/ml） and Immunohistochemistry （2.5 – 5.0 ug/ml）.
免疫原
Synthetic peptide selected from the C-terminal region of human GCK
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的GCK （Glucokinase）蛋白發生免疫反應.
保存建議
提供的GCK （Glucokinase）抗體產品為100 ug 無色溶液，其濃度為0.25 mg/ml，溶解于包含有0.09% （W/V） 疊氮鈉的PBS緩沖液中.請將該GCK （Glucokinase）抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
