Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.
應用類型
Western blotting （0.5-4 ug/ml） and in Immunohistochemistry （10-20 mg/ml）. ）. However， the optimal conditions should be determined individually. The affinity purified antibody mainly detects the 26 kDa fully cleaved fragment and the 35-43 kDa intermediate of caspase-8 in apoptotic samples.
免疫原
Synthetic peptide surrounding the cleavage site of human Caspase-8
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的Caspase-8蛋白發生免疫反應.
保存建議
提供的Caspase-8抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Caspase-8抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
