Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS，Nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
應用類型
The antibody can be used for ELISA （0.25 ug/ml）， Western Blotting （2.5 – 5.0 ug/ml）， Immunohistochemistry （2.5 – 5.0 ug/ml） and Immunofluorescence （5 - 25 ug/ml）.
免疫原
Synthetic peptide selected from the N-terminal region of human Nestin
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的Nestin蛋白發生免疫反應.
保存建議
提供的Nestin抗體產品為100 ug 無色溶液，其濃度為0.25 mg/ml，溶解于包含有0.09% （W/V） 疊氮鈉的PBS緩沖液中.請將該Nestin抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
