Bid，a BH3 domain-containing proapoptotic Bcl-2 family member，is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity，which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies，Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
應用類型
Western blot analysis （0.5-4 ug/ml）， immunoprecipitation （5-10 ug/ml）， and Immunohistochemistry （20-40 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects 22 kDa human Bid
免疫原
Synthetic peptide surrounding amino acid 37 of human Bid
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的Bid蛋白發生免疫反應.
保存建議
提供的Bid抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Bid抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
