IKK （IkB kinase- or IKK-1） is part of a large protein complex responsible for the inducible phosphorylation of IkB proteins. Human IKK- is an 85 kDa peptide that has been shown to activate NFkB by phosphorylation of IkB proteins. IKK interacts with its upstream kinase，NIK，and its downstream substrate，the IkB proteins. Mutations of IKK in its kinase domain lead to a dominant-negative phenotype that suppresses TNF- and IL-1B induced NFkB activation.
應用類型
Western blot （at 0.5-1 ug/ml） and immunoprecipitation （4-8 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
E. coli expressed recombinant human IKKa
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的IKKa/IKK-1蛋白發生免疫反應.
保存建議
提供的IKKa/IKK-1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該IKKa/IKK-1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
