Human perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells,but increases in resting XD8+ cytotoxic cells upon activation.
應用類型
Immunoprecipitation (2-8 ug/ml), Flow cytometry (2-8 ug/ml), Immunohistochemistry (2-8 ug/ml for both frozen and paraffin sections), Immunocytochemistry (2-8 ug/ml),. However, the optimal conditions should be determined individually. Recognizes the 70 kDa perforin in human and primate.
免疫原
Purified granules from human YT lymphoma cells
來源宿主
小鼠
反應性
經檢測驗證,可與人種屬來源的Perforin蛋白發生免疫反應.
保存建議
提供的Perforin抗體產品為100 ug 無色溶液,其濃度為1.0 mg/ml,溶解于包含有1 mg/ml BSA和1.5 mM硫柳汞和50%甘油.的PBS緩沖液中.請將該Perforin抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.