Cathepsin L is a normal lysosomal cysteine endoproteases that is expressed in all cells. The latent 39 kDa pro-Cathepsin L must be processed by cleavage of the 96 amino acid pro-peptide，to generate the active two-chain form made up of 21 and 5 kDa subunits. Mature Cathepsin L is normally localized in the lysosomes where it plays a major role in intracellular protein catabolism. The fact that Cathepsin L is also capable of degrading extracellular matrix proteins suggests that it may play a role in invasion and metastasis and could be a useful prognostic indicator for cancer.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Jurkat cell lysate （Cat.# 2401-100） can be used as a positive control. Detects both the proenzyme and the mature form of cathepsin L
免疫原
Synthetic peptide surrounding amino acid 223 of human Cathepsin L
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的Cathepsin L 蛋白發生免疫反應.
保存建議
提供的Cathepsin L 抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.1% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Cathepsin L 抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
