NIK （NF-kB-inducing kinase） is a member of the MAP kinase kinase kinase family that binds TRAF2 and stimulates NF-kB activity. NIK was initially isolated from a human B cell cDNA library and contains 795 amino acids with an apparent molecular weight of slightly more than 97 kDa on SDS gel. NIK is a serine/threonine kinase and its kinase activity contributes to IkB phosphorylation. The carboxyl terminal segment of NIK binds TRAF2. A mutant NIK with intact carboxyl terminus but without the two lysine residues at its catalytic domain serves as a dominant-negative inhibitor for NF-kB activation. NIK also interacts with TRAF6 and mediates IL-1-induced NF-kB activation.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually
免疫原
Synthetic peptide surrounding amino acid 927of human NIK
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、狗、倉鼠種屬來源的NIK蛋白發生免疫反應.
保存建議
提供的NIK抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該NIK抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
