Bcl-2 (B-cell leukemia/lymphoma 2) gene was discovered by walking the chromosomal breakpoints t(14;18). This gene consists of two exons and several transcripts that encode two different protein products,Bcl-2 and Bcl-2B. Both proteins are approximately 26-30 kDa and differ at their C-terminal region. Bcl-2 suppresses apoptosis (programmed cell death) and this property appears to be modulated by homodimerization or association with Bax and Bad,two cell death promoters
應用類型
Western blot (1-4 ug/ml), Immunoprecipitation, Immunohistochemistry, and Flow cytometry. However the optimal conditions should be determined individually. Recognize the 26 kDa human Bcl-2
免疫原
Synthetic peptide corresponding to resides surrounding a.a. 44 of Human Bcl-2
來源宿主
小鼠
反應性
經檢測驗證,可與人種屬來源的Bcl-2 蛋白 發生免疫反應.
保存建議
提供的Bcl-2 抗體 產品為100 ug 無色溶液,其濃度為0.5 mg/ml,溶解于包含有1 mg/ml BSA和1.5 mM 疊氮鈉和50%甘油的PBS緩沖液中.請將該Bcl-2 抗體 產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.