Nestin is a type IV intermediate filament protein expressed by neuroepithelial stem cells and which has been proposed to represent a marker for putative islet stem cells. It is a high molecular weight protein with a terminus greater than 500 residues. Nestins are expressed by several types of cells during development，mostly in dividing cells of the Central Nervous System，Peripheral Nervous System and myogenic tissues. It also represents the progenitor population of neural stem cell origin. The role of nestin in dynamic cells，particularly structural organization of the cell，appears strictly regulated by phosphorylation，especially its integration into heterogeneous intermediate filaments together with vimentin or -internexin. Nestin has recently received attention as a marker for detecting newly formed endothelial cells.
應用類型
Western blot analysis （3-5 ug/ml） and IHC-paraffin （5 ug/ml）.
免疫原
Amino acids corresponding to 1450-1500 of human Nestin
來源宿主
兔
反應性
經檢測驗證，可與人、黑猩猩種屬來源的Nestin蛋白發生免疫反應
保存建議
提供的100 ug Nestin抗體產品為無色溶液其濃度為0.5 mg/ml溶解于包含有0.05% BSA和0.05%疊氮化鈉的PBS緩沖液中.請將該Nestin抗體產品置于-20 °C冷凍保存，建議分裝使用以避免反復凍融.
