Tumor necrosis factor- （TNF-） is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF- is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-. TNF- converting enzyme （TACE） protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bond metalloprotease-disintegrin in the family of mammalian ADAM （for a disintegrin and metalloprotease）. TACE also processes other cell surface proteins，including TNF receptor，TGF-，L-selectin adhesion molecule，and alpha-cleavage of amyloid protein precursor （APP）. TACE mRNA is expressed in a variety of human and murine tissues.
應用類型
Western blotting （1-2 ug/ml）. However， the optimal conditions should be determined individually. The immunoaffinity purified antibody detects 80-130 kDa bands， which may represent mature protein， precursor and glycosylated TACE. HeLa and Jurkat cell lysate can be used as positive control.
免疫原
Synthetic peptide surrounding amino acid 810 of human TACE
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的TACE蛋白發生免疫反應.
保存建議
提供的TACE抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該TACE抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
