The MBD family of proteins includes MeCP2，MBD1，MBD2，MBD3 and MBD4. MBD2 is highly similar to MBD3 but only MBD2 targets the MeCP1 complex to methylate DNA. MBD2 has two isoforms，MBD2a and MBD2b. MBD2a is a 414 amino acids protein，whereas MBD2b lacks the 152 amino acids N-terminal extension. MBD2a interacts with RNA helicase A to enhance CREB-dependent gene exptression.
應用類型
The antibody can be used in Western Blot analysis （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. Blocking peptide （Cat.# 3232BP-50） is available separately.
免疫原
Synthetic peptide surrounding amino acid 164 of rat MBD2a
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的MBD2a蛋白發生免疫反應
保存建議
提供的100 ug MBD2a抗體產品為無色溶液其濃度為0.5 mg/ml溶解于包含有30%甘油， 0.5% BSA， 5 mM EDTA和0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該MBD2a抗體產品置于-20 °C冷凍保存，建議分裝使用以避免反復凍融.
