Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form，LC3-I is processed and recruited to autophagosomes，where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.
應用類型
Western blot, IF
免疫原
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的LC3蛋白發生免疫反應.
保存建議
提供的LC3 (APG8A) (Isoform A specific)抗體產品為無色液體.建議使用冰袋運輸，收到產品后，請-20 °C儲存.該產品有效期為12個月.
