MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment. Dominant-negative form of MyD88 attenuates IL-1R-mediated NF-?B activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor，which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18. MyD88 is a general adaptor protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.
應用類型
Western Blot analysis （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. Blocking peptide （Cat.# 3244RBP-50） is available separately.
免疫原
Synthetic peptide surrounding amino acid 283 of rat MyD88
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、猴種屬來源的MyD88 蛋白發生免疫反應
保存建議
提供的100 ug MyD88 抗體產品為無色溶液其濃度為0.5 mg/ml溶解于包含有30%甘油， 0.5% BSA， 5 mM EDTA和0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該MyD88 抗體產品置于-20 °C冷凍保存，建議分裝使用以避免反復凍融.
