Human p21-GTPase-activated protein kinase 1 （PAK1） is a functional homolog of STE20 of Saccharomyces cerevisiae. PAK1 is a downstream effector of the Rho-family small GTPases Rac and Cdc42. A serine/threonine kinase，PAK1 induces the formation of filopodia and lamellipodia in Swiss 3T3 cells. PAK1 inhibits myosin light chain kinase，and appears to play a role in the regulation of apoptosis by phosphorylating the death agonist Bad. Membrane targeting of PAK1 induces neurite outgrowth. PAK1 may also regulate NFkB activation.
應用類型
Western blotting （0.5-4 ug/ml） and immunoprecipitation （10-20 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
Recombinant human PAK1
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的PAK1蛋白發生免疫反應.
保存建議
提供的PAK1抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該PAK1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
