Gi2 is a 40-41 kDa alpha-subunit of heterotrimeric protein that shares sequence homology with Gi3 and Gi1. All these G proteins can be ADP-ribosylated at the carboxy terminal Cys of the CAAX sequence，interfering with receptor coupling. Gi2 is expressed widely in tissues and cell lines. Activation of PLC-B by Gi-coupled receptors is the result of release of GBg subunits from Gi. Gi2 regulates potassium channel activation. Mutation at Arg179 has been identified in tumors. This and the Q205L mutant inhibit adenylyl cyclase.
應用類型
Western blot （at 0.5-4 ug/ml） and immunoprecipitation. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 229 of human Gai2
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的Gai2蛋白發生免疫反應.
保存建議
提供的Gai2抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Gai2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
