IkB-alpha protein is a NF-kB inhibitory protein. It binds NF-kB and retains the complex in the cytoplasm，thereby preventing it from entering into nucleus and functioning as a transcription factor. Upon receiving a variety of signals，IkB-alpha undergoes phosphorylation at serine residues by an ubiquitin-dependent protein kinases and finally degraded by the proteasome. Free NF-kB then binds DNA and affects gene expression.
應用類型
Western blot （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Cross-reactivity to IkB-alpha of other species has not been determined.
免疫原
E. coli expressed mouse IkBa
來源宿主
兔
反應性
經檢測驗證，可與小鼠種屬來源的IkBa蛋白發生免疫反應.
保存建議
提供的IkBa抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該IkBa抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
