TLR4 （Toll-like receptor 4） is a mammalain homologue of the Drosophila toll protein. The human TLR4 is a type-1 transmembrane protein containing 799 amino acid residues. The extracellular domain has leucine-rich repeats and the intracellular domain is similar to that of IL-1 receptor. TLR4 is a receptor for lipopolysaccharide （LPS）. In mice that are genetically tolerant to endotoxin，mutations have been identified in the intracellular domain of TLR4 that abolish LPS responsiveness. TLR4 uses a mechanism similar to that of the IL-1 receptor for signal transduction that leads to activation of NF-kB.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes ~92 kDa TLR4 of rat origin. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to residues surrounding amino acids 819 of rat TLR4
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的TLR4蛋白發生免疫反應.
保存建議
提供的TLR4抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該TLR4抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
