Akt，also referred to as PKB and RAC，is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis，although the pathways involved have not been identified. Akt is activated by phospholipid binding and phosphorylation within the activation loop at Thr308 and within the C-terminus at Ser473.
應用類型
Western blotting （0.5-4 ug/ml） and immunoprecipitation （10-20 ug/ml）. However， the optimal conditions should be determined individually. The phospho-Akt （Ser473） antibody detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites.
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Ser473 of mouse Akt
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Phospho-Akt蛋白發生免疫反應.
保存建議
提供的Phospho-Akt抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS，緩沖液中.請將該Phospho-Akt抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
