Caspase-8，a member of the caspase-family of proteases，plays a key role in mediating Fas （CD95） and TNF induced apoptosis. Caspase-8 is synthesized as inactive pro-enzyme and activation of the enzyme involves proteolytic cleavage that leads to the release of the active p18 and p10 subunits. Activated caspase-8 is able to cleave and activate downstream caspases，such as caspase-3，-6，-7 and a death agonist member of the Bcl-2/Bcl-xL family，Bid，leading to apoptosis.
應用類型
Western blotting （0.5-4 ug/ml） and immunoprecipitation （10 ug/ml）. However， the optimal conditions should be determined individually. Detects the 10 kDa small subunit of caspase-8. The antibody does not detect the full-length of caspase-8.
免疫原
Synthetic peptide derived from the amino-terminal sequence of p10 of human caspase-8
來源宿主
小鼠
反應性
經檢測驗證，可與人種屬來源的Caspase-8 （Active）蛋白發生免疫反應.
保存建議
提供的Caspase-8 （Active）抗體產品為100 ug 無色溶液，其濃度為0.2 ug/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS緩沖液中.請將該Caspase-8 （Active）抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
