Bad is a member of the Bcl-2 family protein that selectively dimerize with Bcl-xL and Bcl-2 resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser 136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-xL and Bcl-2. Recently，Akt has also been shown to promote cell survival via its ability to phosphorylate Bad at Ser136.
應用類型
Western blot analysis （1-2 ug/ml） and immunoprecipitation （5-20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects 23 kDa Bad only when phosphorylated at Ser112.
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Ser112 of mouse Bad
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Phospho-Bad蛋白發生免疫反應.
保存建議
提供的Phospho-Bad抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Phospho-Bad抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
