The forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family，three members （FKHR，AFX，AND FKHRL1） have sequence similarity to the nematode homolog Daf 16，recently shown to mediate signaling via a pathway involving IGFR1，P13-K and Akt. There are three potential Akt phosphorylation sites on the FKHR proteins. One site，FKHR Ser256 is found in the Forkhead domain and the other two are outside this domain. Recent studies show that phosphorylation of FKHR family members by Akt promotes cell survival. Phosphorylation regulates FKHR nuclear translocation and target gene transcription. Insulin also stimulates FKHR phosphorylation at Ser253.
應用類型
Western blotting （1-2 ug/ml）. However， the optimal concentrations should be determined individually. Phospho-AFX （Ser193） antibody detects only the phosphorylated Ser193 of AFX and does not react with nonphosphorylated AFX.
免疫原
Synthetic peptide corresponding to residues surrounding Ser193 of human FoxO4/AFX
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的Phospho-FoxO4/AFX蛋白發生免疫反應.
保存建議
提供的Phospho-FoxO4/AFX抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02% 疊氮鈉的PBS緩沖液中.請將該Phospho-FoxO4/AFX抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
