Bid，a BH3 domain-containing proapoptotic Bcl-2 family member，is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity，which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies，Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
應用類型
Western blot analysis （0.5-5 ug/ml） also works well in immunoprecipitation （20 ug/ml） and Immunohistochemistry （20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects the full length （22 kDa） mouse Bid. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 45 of mouse Bid
來源宿主
兔
反應性
經檢測驗證，可與小鼠種屬來源的Bid蛋白發生免疫反應.
保存建議
提供的Bid抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Bid抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
